Rodent Brain Slices

Rodent brain slices

We are able to perform electrophysiological recordings on rodent acute brain slices from any brain regions, such as: 

  • Cortex 
  • Hippocampus  
  • Striatum 
  • Locus Cœruleus 
  • Thalamus 
  • Amygdala 
  • Dorsal Raphe 
  • Periaqueductal gray Matter 
  • Cerebellum 
  • Spinal Cord 
  • Substancia Nigra 
  • Dorsal Vagal Nucleus 
  • Ventral Tegmental Area 
Rodent brain slices in a dish

PROTOCOLS AVAILABLE

PATCH CLAMP
Passive membrane properties

– Input resistance
– Membrane capacitance
– Access resistance
– Resting Membrane Potential (RMP)
PATCH CLAMP
Active membrane properties

– Rheobase
– Spike threshold
– Spike Amplitude
– Firing frequency
– Voltage-gated ion channels
– Ligand gated ion channels
– GPCR modultation
PATCH CLAMP
Synaptic transmission and plasticity

– Evoked responses
– Miniature currents
– Spontaneous currents
MULTI ELECTRODE ARRAY & HD MEA
– Spontaneous firing activity
– Short term synaptic plasticity
– Long term synaptic plasticity

CALCIUM IMAGING

– Firing activity on small neuronal subpopulation

RODENT BRAIN SLICES SAMPLE DATA

MEA – Effect of Carbachol – a cholinergic receptors agonist – on rodent brain slices firing activity.Graphs showing carbachol increasing firing activity

Raw traces showing effects of Carbachol reduced by pirenzepine

  • Carbachol – a cholinergic receptors agonist – strongly increased the firing activity. Carbachol effect stabilized over the 10 first minutes of exposure and then remained steady until the end of the recording session (over 50 minutes).
  • Pirenzepine – a selective M1 antagonist – reduced the effect of carbachol (right-shift of the dose-response curve).