Human Brain Slices

Ex vivo human brain slices

Investigating your compound’s activity on human neurons is a key step to help validate early the translation of rodent data to human.  

We usually have access to brain tissue coming from two brain regions: 

  • Neocortex 
  • Hippocampus

We can perform patch clamp recordings from: 

  • Pyramidal neurons 
  • Dentate gyrus granule neurons 
  • Interneurons
Human brain slice on vibratome - hippocampus slice

We source our human brain tissue thanks to a partnership with the Bordeaux University Hospital. Human brain tissue is collected on human donors (brain resection from epileptic patients). 


Active membrane properties
– Resting Membrane Potential (RMP)
– Sag
Passive membrane properties
– Rm – Input resistance
– Cm – Membrane capacitance
Single AP properties
– Rheobase
– Threshold
– Amplitude
– Halfwidth
– Latency
– fAHP
Repetitive firing properties
– Firing rate
– Accommodation
– sAHP


Spontaneous EPSC

SAMPLE DATA – Human L2/3 Cortex pyramidal neuron activity

Human L2/3 Cortex pyramidal neuron firing activity

Increasing current intensities are injected into the neuron.

The below graphs shown for several current intensities, the firing activity as a function of time.

L2 3 Cortex pyramidal neuron firing activity

Human brain pyramidal neuron approached by a patch pipette

Input / Output curve
Shows the overall firing activity of the L2/3 Cortex pyramidal neuron recorded.

L2 3 Cortex pyramidal neuron I O curve
Vm = -75 mV (RMP), N=1
I O Curve PN Human Neurons
Vm = -50 mV, N=1








Passive & Active membrane properties of the L2/3 cortex pyramidal neuron  

Passive and active membrane properties L2 3 Cortex pyramidal neuron

SAMPLE DATA – Kv.7 channel activator Retigabine in cortical pyramidal neurons

Retigabine graph 10 microM human brain slice







(Left – Vehicule // Right –
100 µM Retigabine)

Dose-inhibiting effect of Retigabine on Pyramidal neuron’s firing at rheobase x2:

Rheobase L2 3 PN Human brain


Dose response curve of retigabine effect on human brain slice