Custom Analysis
Patch Clamp – Action Potential Analysis
Presented Calculation
RMP: Sweep 1
Rm: Mean of sweep 1 and 3
Spike threshold: At rheobase
Spike amplitude: At rheobase
Half-width: At rheobase
Decay-time: At rheobase
Firing: All sweeps
Latency: All sweeps (minimal is plotted)
First ISI: All sweeps
Not Shown
Last ISI + Adaptation: All sweeps
AHP fast: At Rheobase
AHP slow: All sweeps
Patch Clamp – Evoked EPSC Analysis
Amplitude: synaptic strength
Area: charge transfer
Rise time: kinetics of channel activation
Decay time: kinetics of channel inactivation
Paired-pulse ratio: short-term plasticity
(amplitude EPSC2/EPSC1)
Patch Clamp – Miniature EPSC Analysis
Amplitude: quantal size
Area: charge transfer
Rise time: kinetics of channel activation
Decay time: kinetics of channel inactivation
Frequency: presynaptic release characteristics