Cloned Channels and Receptors
Cloned channels and receptor
We can perform recordings on:
- Stable cell lines
- Transfected cells
PROTOCOLS AVAILABLE
PATCH CLAMP
Passive membrane properties
– Input resistance
– Membrane capacitance
– Access resistance
– Resting Membrane Potential (RMP)
Passive membrane properties
– Input resistance
– Membrane capacitance
– Access resistance
– Resting Membrane Potential (RMP)
PATCH CLAMP
Active membrane properties
– Rheobase
– Spike threshold
– Spike Amplitude
– Firing frequency
– Voltage-gated ion channels
– Ligand gated ion channels
– GPCR modultation
Active membrane properties
– Rheobase
– Spike threshold
– Spike Amplitude
– Firing frequency
– Voltage-gated ion channels
– Ligand gated ion channels
– GPCR modultation
PATCH CLAMP
Synaptic transmission and plasticity
– Evoked responses
– Miniature currents
– Spontaneous currents
Synaptic transmission and plasticity
– Evoked responses
– Miniature currents
– Spontaneous currents
HD MEA
– Spontaneous firing activity
– Short term synaptic plasticity
– Long term synaptic plasticity
CALCIUM IMAGING
– Firing activity on small neuronal subpopulation
– Spontaneous firing activity
– Short term synaptic plasticity
– Long term synaptic plasticity
CALCIUM IMAGING
– Firing activity on small neuronal subpopulation