Human iPSC-Derived Neurons
NOVEL TOOL FOR TRANSLATIONAL RESEARCH: HUMAN IPSC-DERIVED NEURONS
Genetic and biochemical manipulation of stem cells has empowered the world scientists to theoretically generate any kind of cells, including any kind of neurons.
We are able to physiologically characterize your cells / evaluate your compound’s efficacy on:
- Proprietary iPSCs (healthy & patient-derived)
- Commercially available iPSCs (healthy & patient-derived)
- Glutamatergic
- Dopaminergic
- GABAergic
- Motor
- Sensory
PROTOCOLS AVAILABLE
PATCH CLAMP
Passive membrane properties
– Input resistance
– Membrane capacitance
– Access resistance
– Resting Membrane Potential (RMP)
Passive membrane properties
– Input resistance
– Membrane capacitance
– Access resistance
– Resting Membrane Potential (RMP)
PATCH CLAMP
Active membrane properties
– Rheobase
– Spike threshold
– Spike Amplitude
– Firing frequency
– Voltage-gated ion channels
– Ligand gated ion channels
– GPCR modultation
Active membrane properties
– Rheobase
– Spike threshold
– Spike Amplitude
– Firing frequency
– Voltage-gated ion channels
– Ligand gated ion channels
– GPCR modultation
PATCH CLAMP
Synaptic transmission and plasticity
– Evoked responses
– Miniature currents
– Spontaneous currents
Synaptic transmission and plasticity
– Evoked responses
– Miniature currents
– Spontaneous currents
CALCIUM IMAGING
– Firing activity on small neuronal subpopulation
HD MEA
– Global firing activity (large neuronal population)
– Pharmacological identification of neuronal subpopulations
– Firing activity on small neuronal subpopulation
HD MEA
– Global firing activity (large neuronal population)
– Pharmacological identification of neuronal subpopulations
HUMAN ISPC-DERIVED NEURONS RECORDING: SAMPLE DATA
Sodium currents