Rodent Brain Neuron Cultures
RODENT BRAIN NEURON CULTURES FOR CNS DRUG DISCOVERY
Rodent brain neuron cultures constitute an easy approach to investigate the effect of compounds on membrane properties (ion channels). This type of assay offers mid-throughput capabilities and small series of compounds profiling.
We provide different brain regions neuronal cultures:
- Hippocampal
- Cortical
- Striatal
- Cerebellar
- …
This approach provides translational, functional data to assess how molecules act on ion channels & GPCRs and modulate their properties.
PROTOCOLS AVAILABLE
PATCH CLAMP
Passive membrane properties
– Input resistance
– Membrane capacitance
– Access resistance
– Resting Membrane Potential (RMP)
Passive membrane properties
– Input resistance
– Membrane capacitance
– Access resistance
– Resting Membrane Potential (RMP)
PATCH CLAMP
Active membrane properties
– Rheobase
– Spike threshold
– Spike Amplitude
– Firing frequency
– Voltage-gated ion channels
– Ligand gated ion channels
– GPCR modultation
Active membrane properties
– Rheobase
– Spike threshold
– Spike Amplitude
– Firing frequency
– Voltage-gated ion channels
– Ligand gated ion channels
– GPCR modultation
PATCH CLAMP
Synaptic transmission and plasticity
– Evoked responses
– Miniature currents
– Spontaneous currents
Synaptic transmission and plasticity
– Evoked responses
– Miniature currents
– Spontaneous currents
CALCIUM IMAGING
– Firing activity on small neuronal subpopulation
HD MEA
– Global firing activity (large neuronal population)
– Pharmacological identification of neuronal subpopulations
– Firing activity on small neuronal subpopulation
HD MEA
– Global firing activity (large neuronal population)
– Pharmacological identification of neuronal subpopulations
RODENT BRAIN NEURON CULTURES SAMPLE DATA
Cultured neurons from Rat Embryonic Cortex with patch clamp electrode and Theta tube for fast agonist application
Block and relief of NMDA current by Ketamine