Our LTP offer
Does your compound modulate LTP?
We have optimized our internal production process of LTP recording to address cognition and neurodegeneration programs with very attractive prices and fast turn-around.
We propose a new offer to evaluate:
- 1 compound / 1 concentration
- 1 compound / 3 concentrations
Long-Term Potentiation (LTP) is one of the main synaptic plasticity mechanisms which are fundamental for basic learning tasks and higher cognitive functions. Multi-Electrode Array (MEA) recordings provide an exceptional macroscopic view of native neuronal network with quick turnaround: ideal to bridge the gap between cellular and in vivo assays, to confirm compound activities and/or investigate their mechanism(s) of action.
Few examples: reference compounds effect on LTP
Materials & Methods
- Experiments carried out with 8 to 12 week-old C57Bl6 mice*
- 350 µM thick hippocampal slices prepared with a McILWAIN tissue chopper
- Hippocampal slices continuously perfused with oxygenated ACSF (bubbled with 95% O2–5% CO2) at the rate of 3 mL/min
Stimulation/Recording protocols and compound application
- Schaffer Collaterals stimulation, recording of evoked-responses (fEPSP) in the CA1 region
- Stimulation intensity set to 40%* of Imax
- Long Term Potentiation Recording:
- 10 minutes in control condition (Baseline)
- 30 minutes* of compound exposure
- LTP triggered by a High Frequency Stimulation (HFS)* of the afferent pathway
- 60 minutes of recording after tetanus (in the continuous presence of the compound)
Number of samples
- Evaluation of 1 concentration: n = 10 slices (n=5 compound-exposed slices + n = 5 control slices)
- Evaluation of 3 concentrations: n = 23 slices (n=5 compound-exposed slices per concentration + n = 8 control slices)