Our LTP offer
Does your compound modulate LTP?
We have optimized our internal production process of LTP recording to address cognition and neurodegeneration programs with very attractive prices and fast turn-around.
We propose a new offer to evaluate:
- 1 compound / 1 concentration
- 1 compound / 3 concentrations
Long-Term Potentiation (LTP) is one of the main synaptic plasticity mechanisms which are fundamental for basic learning tasks and higher cognitive functions. Multi-Electrode Array (MEA) recordings provide an exceptional macroscopic view of native neuronal network with quick turnaround: ideal to bridge the gap between cellular and in vivo assays, to confirm compound activities and/or investigate their mechanism(s) of action.
Few examples: reference compounds effect on LTP
EVALUATION OF NMDA NON-COMPETITIVE ANTAGONIST ON Long Term Potentiation – Illustrated is the dose-dependent inhibition of Long Term Potentiation by the non-competitive NMDA receptor antagonist, Memantine, in the CA1 region of rat hippocampal slices.
REVERSAL OF MK-801-LTP DISRUPTION BY D-SERINE – LTP induced by Theta-Burst Stimulation (TBS) is largely decreased by MK-801 (with an approximate IC50 of 9 µM). Pre-exposure with 1 mM D-Serine prevents the MK-801-induced LTP disruption.
EVALUATION OF NMDA COMPETITIVE ANTAGONIST ON LTP – Illustrated is the dose-dependent inhibition of Long Term Potentiation by the competitive NMDA receptor antagonist, D-AP5, in the CA1 region of rat hippocampal slices.
EVALUATION OF DOPAMINERGIC AGONIST ON LTP – Pre-exposure with 30 µM Dopamine largely increased Long Term Potentiations amplitude in the CA1 region of rat hippocampal slices.
Materials & Methods
- Experiments carried out with 8 to 12 week-old C57Bl6 mice*
- 350 µM thick hippocampal slices prepared with a McILWAIN tissue chopper
- Hippocampal slices continuously perfused with oxygenated ACSF (bubbled with 95% O2–5% CO2) at the rate of 3 mL/min
Stimulation/Recording protocols and compound application
- Schaffer Collaterals stimulation, recording of evoked-responses (fEPSP) in the CA1 region
- Stimulation intensity set to 40%* of Imax
- Long Term Potentiation Recording:
- 10 minutes in control condition (Baseline)
- 30 minutes* of compound exposure
- LTP triggered by a High Frequency Stimulation (HFS)* of the afferent pathway
- 60 minutes of recording after tetanus (in the continuous presence of the compound)
Number of samples
- Evaluation of 1 concentration: n = 10 slices (n=5 compound-exposed slices + n = 5 control slices)
- Evaluation of 3 concentrations: n = 23 slices (n=5 compound-exposed slices per concentration + n = 8 control slices)