Calcium Imaging

CALCIUM IMAGING: FUNCTIONAL IMAGING

WHAT IS CALCIUM IMAGING?

Calcium plays a key role in cell signaling: the movement of Calcium ions provoque a change in cells membrane potential, which is partly responsible for neuronal signals transmission. Calcium signaling regulates a wide range of physiological properties of cells.

 

Calcium Imaging is an essential technique to evaluate Calcium ions role in functioning neurons. The Calcium Imaging technique uses a fluorescent “dye” that responds to the binding of Ca+ ions and changes its fluorescent properties, allowing optical measurement of Calcium concentration shift within neurons and neuronal tissue.

Hamamatsu recording camera for Calcium Imaging
Hamamatsu recording device used at Neuroservice

Thanks to Calcium Imaging, you can either measure individual cell or global neuronal network Calcium dynamics at a very high resolution. Calcium Imaging analysis tools allow to circle regions of interest in the studied neuronal culture, so that AP firing can be measured locally.

Calcium imaging is a mid-throughput technique.

WHAT FOR?

We can perform Calcium Imaging on:

  • Cloned channels and receptors
  • Rodent brain neuron cultures
  • Rodent brain slices
  • Human iPSC-Derived neurons
  • Rodent DRG sensory neurons cultures.
    Calcium imaging on rodent DRG sensory neurons can help investigate at the earlier stages of the drug discovery process the pharmacology of compounds developed for Pain pathologies. If you want to learn more about how we can provide you with a complementary study on human DRG sensory neurons, please visit www.neuroservices-alliance.com.
Rodent DRG cells in imaging dishes
Rodent DRG cells in imaging dishes at 7 days in vitro

 

ADVANTAGES OF CALCIUM IMAGING

in vitro functional imaging of tissue
Select areas of interest to study
Quantify subpopulations of responsive neurons
Mid-throughput solution

NEURONAL CULTURES RECORDINGS

Calcium imaging helps document and understand compounds activity at the single neuron level or neuronal network level.

Measurements

  • Firing activity of small neuronal subpopulations
  • Capsaicin-induced firing activity

Calcium Imaging - Firing activity capcaisin Calcium Imaging - Firing activity induced by capcaisin

  • 30 nM capcaisin (1min) elicits a calcium response in 50% of DRG sensory neurons
  • 60 nM KCl used as a positive control to identify healthy, excitable DRG sensory neurons

SAMPLE STUDIES

More information coming soon.