Patch-Clamp

PATCH-CLAMP: SINGLE NEURON ELECTROPHYSIOLOGY

WHAT IS PATCH CLAMP?

Patch Clamp recordings are performed with borosilicate electrodes of 1-2 μm tip diameter. A pipette containing an electrolyte solution is tightly sealed onto the neuronal membrane and isolates a part of the membrane (patch) electrically.

Currents fluxing through the channels in this patch flow into the pipette and can be recorded by an electrode that is connected to a highly sensitive differential amplifier. The pipette tip makes a giga-ohm seal contact with the neuronal membrane.

Patch Clamp recordings can be performed in different configurations: 

  • Single channel (as described above)
  • Whole-cell (break of the cell membrane and access to the whole membrane conductancies)
patch-clamp-graphic neuroservice

WHAT FOR?

This technology provides the highest resolution for electrophysiological recordings from single neuron down to single channel thanks to the different recording configurations (whole-cell, perforated-patch, cell-attached, outside-out).

The Patch Clamp technique is dedicated to the investigation of Mechanism of Action to answer very precise physiological and pharmacological questions.

WHAT ARE THE ADVANTAGES OF PATCH-CLAMP RECORDING?

In vitro evaluation of compounds under the best physiological conditions
Refined investigations of compounds’ effect on single proteins/receptors
Capabilities to address physiological/pharmacological questions in all CNS structures
High resolution in vitro data

TWO PHYSIOLOGICAL RECORDINGS LEVEL: CELL & SLICE

Patch-clamp recordings is essential to document and understand compounds activity at the single neuron level.

Measurements

  • Voltage-gated ion channels
  • GPCR modulation of voltage-gated ion channels
  • Ligand-gated ion channels

CELLS

Rodent Dorsal Root Ganglia neurons

Dorsal Root Ganglion neurons are peripheral sensory neurons essential for Pain preclinical investigations.
We use whole-cell patch clamp recordings on rodent DRG neurons to assess pharmacological agents targeting ion channels.

In addition, we can record DRG neurons from neuropathic rodents to address compound properties specifically developed for neuropathic pains.

This approach provides translational, functional data to assess how molecules act on ion channels and alter their properties.

DRG neurons are a powerful and versatile drug discovery tool for analgesic therapeutics.

Patch clamp DRG Neuron rodent
Rodent brain neuron cultures

Neuron cultures constitute an easy approach to investigate the effect of compounds on membrane properties (ion channels). This type of assay offers mid-throughput capabilities and small series of compounds profiling.

We can record from cortical neurons and hippocampal neurons.

Patch clamp on rodent brain cultured cell
Cloned channels and receptors
  • Stable cell lines
  • Transfected cell lines
Patch clamp recording on cloned channels and receptors
Human iPSC-derived neurons

We are able to physiologically characterise your cells / evaluate your compound’s efficacy on:

  • Proprietary iPSCs
  • Commercially available iPSCs

Measurements

  • Membrane properties
  • Voltage-gated ion channels
  • Ligand-gated ion channels
  • GPCR modulation

 

patch clamp on ipsc derived neuron

SLICES

Rodent brain slice

 

Patch clamp on rodent brain slice
Human brain slice

Thanks to our partnership with neurosurgeons from the CHU of Bordeaux, we have access to human brain tissue resulting from brain resection surgeries.

We are able to perform patch clamp recordings on different cell types:

  • Pyramidale neurons
  • Dentate gyrus / granule neurons
  • Interneurons

We provide different recording types:

  • Spontaneous synaptic activity
  • Intrinsic properties
  • Membrane properties
  • Single AP properties
  • Trains of APs
Patch clamp recording on human hippocampal neuron

NEUROSERVICE’S PATCH CLAMP TEAM

Neuroservice's Patch clamp laboratory

SAMPLE STUDIES

EVALUATION OF ALPHA7 NICOTINIC RECEPTOR AGONIST ON GABAERGIC SYNAPTIC ACTIVITY

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HIPPOCAMPAL CULTURE EVALUATION OF COMPOUND ON NMDA RECEPTORS

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