News

Our LTP offer

Our LTP offer

News
30.10.2015

#LTP, #MEA

Does your compound modulate LTP?

FAST TURN-AROUND

We have optimized our internal production process of LTP recording to address cognition and neurodegeneration programs with very attractive prices and fast turn-around.

We propose a new offer to evaluate:

  • 1 compound / 1 concentration
  • 1 compound / 3 concentrations

Long-Term Potentiation (LTP) is one of the main synaptic plasticity mechanisms which are fundamental for basic learning tasks and higher cognitive functions. Multi-Electrode Array (MEA) recordings provide an exceptional macroscopic view of native neuronal network with quick turnaround: ideal to bridge the gap between cellular and in vivo assays, to confirm compound activities and/or investigate their mechanism(s) of action.

Few examples: reference compounds effect on LTP

EVALUATION OF NMDA NON-COMPETITIVE ANTAGONIST ON Long Term Potentiation – Illustrated is the dose-dependent inhibition of Long Term Potentiation by the non-competitive NMDA receptor antagonist, Memantine, in the CA1 region of rat hippocampal slices.


REVERSAL OF MK-801-LTP DISRUPTION BY D-SERINE – LTP induced by Theta-Burst Stimulation (TBS) is largely decreased by MK-801 (with an approximate IC50 of 9 µM). Pre-exposure with 1 mM D-Serine prevents the MK-801-induced LTP disruption.


EVALUATION OF NMDA COMPETITIVE ANTAGONIST ON LTP – Illustrated is the dose-dependent inhibition of Long Term Potentiation by the competitive NMDA receptor antagonist, D-AP5, in the CA1 region of rat hippocampal slices.


 EVALUATION OF DOPAMINERGIC AGONIST ON LTP – Pre-exposure with 30 µM Dopamine largely increased Long Term Potentiations amplitude in the CA1 region of rat hippocampal slices.

Materials & Methods

Slices preparation

  • Experiments carried out with 8 to 12 week-old C57Bl6 mice*
  • 350 µM thick hippocampal slices prepared with a McILWAIN tissue chopper
  • Hippocampal slices continuously perfused with oxygenated ACSF (bubbled with 95% O2–5% CO2) at the rate of 3 mL/min

Stimulation/Recording protocols and compound application

  • Schaffer Collaterals stimulation, recording of evoked-responses (fEPSP) in the CA1 region
  • Stimulation intensity set to 40%* of Imax
  • Long Term Potentiation Recording:
  1. 10 minutes in control condition (Baseline)
  2. 30 minutes* of compound exposure
  3. LTP triggered by a High Frequency Stimulation (HFS)* of the afferent pathway
  4. 60 minutes of recording after tetanus (in the continuous presence of the compound)

Number of samples

  • Evaluation of 1 concentration: n = 10 slices (n=5 compound-exposed slices + n = 5 control slices)
  • Evaluation of 3 concentrations: n = 23 slices (n=5 compound-exposed slices per concentration + n = 8 control slices)
*Species (mice or rats), stimulation intensity, tetanus protocol and time of compound exposure can be adjusted under request without additional fees (assuming the recording session duration remains unchanged)

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