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Tests related to Toxicity effects

The NEUROTOX PACKAGE: the right & the fast answer to any neuro-toxicological issue.
Evaluation of the absence of effects of 1 compound (at 2 concentrations) on 5 main CNS functions:

Spontaneous neuronal firing
Basal synaptic transmission
Short-Term synaptic plasticity (Paired-Pulse Facilitation)
Potentiation of Long-Term synaptic plasticity (Long-Term Potentiation)
Inhibition of Long-Term synaptic plasticity (Long-Term Depression)

WITH:

Multi-Electrode Array recordings
Mice or rats
Acute Hippocampal slices
3 slices/concentration

1 week to get the results: effect or not effect

Any effect detected: further investigation proposal

Download the Neurotox Package

ADDITIONAL STANDARDIZED ASSAYS:

Toxic effects

Code

Name

Target

Pharm. Ref.

Biology

PDF

Interaction with fast sodium channels or AP

SAP-001

Spontaneous Action Potentials of Purkinje Neurons

Sodium channels

TTX and lidocaine

Action Potentials propagation

Interaction with fast sodium channels or AP

SAP-002

Evoked Spikes in the CA3/CA1 region

Sodium channels

TTX

Action Potentials propagation

Modification of excitatory/ inhibitory neurotransmission weight

SST-001

Basal Synaptic Transmission (Schaeffer Collaterals stimulation)

AMPA/Kainate receptors

NBQX/CNQX

Glutamate-mediated neurotransmission under GABA tone

Modification of short-term synaptic plasticity

SST-002

Paired-Pulse Facilitation in CA1 region

Presynaptic receptors (GPCR)

Carbachol

Short-term pre-synaptic plasticity

Interaction with long-lasting plasticity potentiation

SST-003

Long-Term Potentiation in CA1 region (100 Hz protocol)

NMDA receptors

D-AP5 / MK801 / memantine

Long-term synaptic plasticity

Interaction with long-lasting plasticity depression

SST-004

Long-Term Depression in CA1 region (1 Hz protocol)

NMDA receptors

D-AP5

Long-term synaptic plasticity

Interaction with long-lasting plasticity potentiation

SST-005

Long-Term Potentiation in CA3 region (100 Hz protocol)

GluR5-containing kainate receptors

On going

Long-Term synaptic plasticity